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NTEGRA Spectra NTEGRA Spectra – AFM-Raman-SNOM system. NT-MDT – AFM-probes, atomic force microscope (AFM, HybriD Mode, STM, SPM, RAMAN, SNOM SPECTRUM SPECTRUM - Automated AFM-Raman-SNOM system for a wide range of applications NTEGRA Spectra II

NTEGRA Spectra

Interdisciplinary research at the nanometer scale:
AFM + Confocal Raman + SNOM + TERS
NTEGRA Spectra – AFM-Raman-SNOM system. NT-MDT – AFM-probes, atomic force microscope (AFM, STM, SPM, RAMAN, SNOM
NTEGRA Spectra Brochure (8 Мб)
Working principle Applications Specifications Downloads Contact Us

Integration of SPM and confocal microscopy/Raman scattering spectroscopy. Owing to Tip Enhances Raman Scattering it allows carrying out spectroscopy/microscopy with up to 10 nm resolution.


Integration: The key to the new sciences
Change happens at interfaces and today’s most exciting changes in microscopy are happening where multiple technologies are interfaced together. NTEGRA Spectra is a prime example, uniting the full power of atomic force microscopy (AFM), confocal Raman and fluorescence microscopy and scanning near-field optical microscopy (SNOM) in one platform. more info

Different configuration of AFM with confocal Raman/Fluorescence microscope


A unique configuration for simultaneous AFM - Raman - TERS* and SNOM imaging of opaque samples

*TERS: Tip Enhanced Raman Scattering, Tip Enhanced Fluorescence etc.


Optimized for simultaneous AFM - Raman - TERS* and SNOM imaging of samples on transparent substrates (living cells, nanoparticles etc.)

*TERS: Tip Enhanced Raman Scattering, Tip Enhanced Fluorescence etc.

Side illumination option

Used to facilitate TERS* measurements on opaque samples

*TERS: Tip Enhanced Raman Scattering, Tip Enhanced Fluorescence etc.

Fiber Scanning Near-field Optical Microscopy (SNOM)

SNOM techniques based on on quartz fiber.


Cantilever Scanning Near-field Optical Microscopy (SNOM)

SNOM techniques based on cantilevers with aperture.

•     Atomic Force Microscopy ( > 30 modes )
•     Confocal Raman / Fluorescence / Rayleigh Microscopy
•     Scanning Near-Field Optical Microscopy ( SNOM / NSOM )
•     Optimized for Tip Enhanced Raman and Fluorescence (TERS, TEFS, TERFS) and scattering SNOM (s-SNOM)
New era of integration 
Optical AFM (NT-MDT) + Raman spectrometer (Renishaw) = Join the best technologies in one system

HybriD Mode™
Ntegra Spectra equipped with new electronics and software allows to combine a recently developed innovative HybriD Mode™ (HD-AFM™ Mode) for nanomechanical proprieties and Raman for chemical imaging of exactly the same area within single measurement session.


Stiffness of HDPE/LDPE polymer sandwich cut by microtome


Overlap of Raman maps: HDPE (red), LDPE (blue)


AFM topography

Image size: 34 × 34 μm
Data from: M. Yanul, S. Magonov, P. Dorozhkin, NT-MDT.



Working principle

Modes: Controlled environment:
  • AFM (mechanical, electrical, magnetic properties, nanomanipulation etc.)
  • White Light Microscopy and Confocal Laser (Rayleigh) Imaging
  • Confocal Raman Imaging and Spectroscopy
  • Confocal Fluorescence Imaging and Spectroscopy
  • Scanning Near-Field Optical Microscopy (SNOM)
  • Tip Enhanced Raman and Fluorescence Microscopy (TERS, TEFS, TERFS)
  • Temperature
  • Humidity
  • Gases
  • Liquid
  • Electrochemical environment
  • External magnetic field


NTEGRA Spectra: description and applications   (7.8 Mb)

  • Graphene, carbon nanotubes and other carbon materials
  • Semiconductor devices
  • Nanotubes, nanowires, quantum dots and other nanoscale materials
  • Polymers
  • Optical device characterization: semiconductor lasers, optical fibers, waveguides, plasmonic devices
  • Investigation of cellular tissue, DNA, viruses and other biological objects
  • Chemical reaction control


Graphene flakes

30x30 um

Ni foil

20x20 um

PC-PVAC film

30x30 um


30x30 um



Confocal Raman/Fluorescence microscopy
AFM/STM: Integration with spectroscopy
Scanning Near Field Optical Microscopy (SNOM)
Optimized for Tip Enhanced Raman Scattering (TERS) and other tip-related optical techniques
Confocal Raman/Fluorescence microscopy
Confocal Raman/Fluorescence/Rayleigh imaging runs simultaneously with AFM (during one sample scan)
Diffraction limited spatial resolution: <200 nm in XY, <500 nm in Z (with immersion objective)
True confocality; push button from software to control the motorized confocal pinhole for optimal signal and confocality
Motorized variable beam expander/collimator: adjusts diameter and collimation of the laser beam individually for each laser and each objective used
Full 3D (XYZ) confocal imaging with powerful image analysis
Hyperspectral imaging (recording complete Raman spectrum in every point of 1D, 2D or 3D confocal scan) with further software analysis
Optical lithography (vector, raster)
AFM/STM: Integration with spectroscopy
Upright and Inverted optical AFM configurations (optimized for opaque and transparent samples correspondingly);
side illumination option
Highest possible resolution (numerical aperture) optics is used simultaneously with AFM: 0.7 NA for Upright, 1.3–1.4 NA for Inverted
AFM/STM and confocal Raman/Fluorescence images are obtained simultaneously (during one scan)
All standard SPM imaging modes are supported (>30 modes) — combined with confocal Raman/Fluorescence
Low noise AFM/STM (atomic resolution)
Vibrations and thermal drifts originating from optical microscope body are minimized due to special design of optical AFM heads
Focus track feature: sample always stays in focus due to AFM Z-feedback; high quality confocal images of very rough or inclined samples can be obtained
Seamless integration of AFM and Raman; all AFM/ Raman/SNOM experiment and further data analysis is performed in one and the same software
Powerful analysis of 1D, 2D and 3D hyperspectral images 
Powerful export to other software (Excel, MatLab, Cytospec etc.)
Extremely high efficiency 520 mm length spectrometer with 4 motorized gratings
Visible, UV and IR spectral ranges available
Echelle grating with ultrahigh dispersion; spectral resolution: 0.007 nm (< 0.1 1/cm)**
Up to 3 different detectors can be installed
  • TE cooled (down to -100 ºC) CCD camera. EMCCD camera is optional — for ultrafast imaging
  • Photon multiplier (PMT) or avalanche photodiode in photon counting mode
  • Photon multiplier for fast confocal laser (Rayleigh) imaging
Flexible motorized polarization optics in excitation and detection channels, cross-polarized Raman measurements 
Fully automated switch between different lasers — with a few mouse clicks
Scanning Near Field Optical Microscopy (SNOM)
Two major SNOM techniques supported: (i) based on quartz fiber probes, (ii) based on silicon cantilever probes
All modes supported: Transmission, Collection, Reflection 
All SNOM signals detected: laser intensity, fluorescence intensity, spectroscopy

SNOM lithography (vector, raster)

Optimized for Tip Enhanced Raman Scattering (TERS) and other tip-related optical techniques
All existing TERS geometries are available: illumination / collection from bottom, from top or from side
Different SPM techniques and TERS probes can be used: STM, AFM cantilever, quartz tuning fork in tapping and shear force modes
Dual scan (for Hot Point Mapping in TERS): scan by sample AND scan by tip / by laser spot
Motorized polarization optics to produce optimal polarization for TERS

AFM-Raman measurements can run in air, in controlled atmosphere or in liquid — all with variable temperature (for Inverted configuration)

Some features listed are optional — not included into basic system configuration
* NT-MDT AFM can be integrated with Renishaw inVia or with NT-MDT spectrometer. Specifications are given for the latter. Renishaw specifications can be found at www.renishaw.com/AFM-Raman
** Exact value of spectral resolution highly depends on how “resolution” is defined


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